Crossbreeding and Backcrossing in the Pyrethroid-Resistant Ladybird Beetle Eriopis connexa (Germar) Determines Resistance in Offspring

Abstract

The conservation of the predatory ladybird beetle, Eriopis connexa (Germar) after its release also relies on its resistance and the performance of its progenies. When resistant individuals are released or evolve in the field through mating with susceptible pairs, we anticipate that they will generate a resistant progeny, inheriting the resistance to lambda-cyhalothrin through an autosomal mechanism. The susceptibility of a field-collected population (EcGA) was characterized and determined by the performance of their progenies generated through mating with a resistant parent (EcViR). We paired virgin adults from the EcGA and EcViR populations, observing how their progenies developed, reproduced, and survived when exposed to dried lambda-cyhalothrin residues applied at field rates. Adults from the EcGA population were ~200-fold more susceptible than those of the EcViR population. Developmental times from larva to adult emergence for EcGA individuals were delayed by approximately 6 days and generated smaller adults compared to EcViR and their progenies. The egg production did not differ across parents and progenies, but females from EcGA and EcGA × EcViR progenies produced 130 more eggs in comparison to EcViR females during the 35-day evaluation period. Exposure to lambda-cyhalothrin resulted in 77.4 to 100% survival for adults from EcViR × EcGA progenies and EcViR parents, while EcGA and the population standard for susceptibility maintained in the laboratory (EcFM) did not survive the insecticide exposure. These findings indicate that field crosses between EcViR and EcGA will improve their progenies’ biological performance compared to the EcViR parents and will maintain a high lambda-cyhalothrin survival rate.