Cross-breed comparisons identified a critical 591-kb region for bovine carcass weight QTL (CW-2) on chromosome 6 and the Ile-442-Met substitution in NCAPG as a positional candidate

Kouji Setoguchi,Takashi Hirano,Masako Furuta,Yoshikazu Sugimoto,Akiko Takasuga,Toshio Watanabe,Tomoko Nagao

doi:10.1186/1471-2156-10-43

Kouji Setoguchi, Takashi Hirano + Show 5 more

Open Access

https://doi.org/10.1186/1471-2156-10-43

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Journal: BMC genetics	Publication Date: Aug 4, 2009
Citations: 139	License type: cc-by

Affiliation: Institute of Livestock and Grassland Science

Abstract

BackgroundGrowth-related traits have been mapped on bovine chromosome 6 (BTA 6) in various bovine breed populations. We previously mapped a significant quantitative trait locus (QTL) for carcass and body weight (CW-2) between 38 and 55 cM on BTA 6 using a Japanese Black half-sib family. Additional QTL mapping studies detected four QTL for body or carcass weight that overlapped with CW-2 in Japanese Black and Japanese Brown half-sib families. To map the region in greater detail, we applied cross-breed comparisons of haplotypes that have been shown to be powerful in canine.ResultsWe used 38 microsatellite markers to search for a shared Q (increasing carcass and/or body weight) haplotype within the 17-cM CW-2 region among five sires. Linkage disequilibrium mapping using maternal alleles of the offspring showed that an 815-kb shared Q haplotype was associated with body or carcass weight in both breeds. The addition of 43 single nucleotide polymorphism (SNP) markers narrowed the region to 591 kb containing 4 genes. The SNP changing Ile-442 to Met in NCAPG (chromosome condensation protein G) was significantly associated with carcass weight (p < 1.2 × 10-11) in a large Japanese Black population as well as in the five families. The Q allele of the SNP was also associated with a larger longissimus muscle area and thinner subcutaneous fat thickness in steers of all five families, indicating that the CW-2 locus is pleiotropic and favorable for marker-assisted selection of beef cattle.ConclusionA 591-kb critical region for CW-2 was identified. The SNP changing Ile-442 to Met in NCAPG (chromosome condensation protein G) can be used as a positional candidate of CW-2 for marker-assisted selection.

Highlights

Growth-related traits have been mapped on bovine chromosome 6 (BTA 6) in various bovine breed populations
Carcass and/or body weight quantitative trait locus (QTL) on BTA 6 were mapped in three Japanese Black half-sib families (Sires A-C) and two Japanese Brown half-sib families (Sires D and E; Figure 1; Additional file 1)
Because the 95% confidence intervals (CI) of the QTL in the other families overlapped with the CW-2 region (38–55 cM), we assumed that these

Summary

Introduction

Growth-related traits have been mapped on bovine chromosome 6 (BTA 6) in various bovine breed populations. We previously mapped a significant quantitative trait locus (QTL) for carcass and body weight (CW-2) between 38 and 55 cM on BTA 6 using a Japanese Black half-sib family. We previously performed bovine quantitative trait locus (QTL) mapping for growth and carcass traits using Japanese Black paternal half-sib families constructed from a commercial population [1,2,3]. In these studies, a carcass weight QTL, CW-1 on bovine chromosome 14 (BTA 14), was detected in five families with significant linkages and successfully narrowed down to a 1.1-Mb region by identical-by-descent mapping [3] and linkage disequilibrium (LD) mapping [4].

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Results

Conclusion