Cross-talk of PGD2 receptors: the DP receptor modulates signaling and trafficking of CRTH2

Miriam Sedej,Wolfgang Platzer,Maria Waldhoer,Ákos Heinemann

doi:10.1186/1471-2210-10-s1-a7

Abstract

Background Prostaglandin (PG) D2 is substantially involved in allergic inflammation and signals via the seven transmembrane (7TM) spanning/G protein-coupled receptors (GPCRs) chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and D-type prostanoid (DP) receptor. Both receptors are co-expressed in eosinophils among other immune cells and have emerged as therapeutic targets in allergic diseases. While a proinflammatory function of the CRTH2 receptor is well recognized, the role of the DP receptor in allergic inflammation, however, remains unclear. As it has been shown for many other 7TM/GPCRs, we believe that intermolecular cross-talk occurs between CRTH2 and DP receptors which might be essential for receptor function and regulation.

Highlights

Prostaglandin (PG) D2 is substantially involved in allergic inflammation and signals via the seven transmembrane (7TM) spanning/G protein-coupled receptors (GPCRs) chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and D-type prostanoid (DP) receptor
While a proinflammatory function of the CRTH2 receptor is well recognized, the role of the DP receptor in allergic inflammation, remains unclear. As it has been shown for many other 7TM/GPCRs, we believe that intermolecular cross-talk occurs between CRTH2 and DP receptors which might be essential for receptor function and regulation
Ca2+ flux in human eosinophils was measured by Fluid Excitation (FLEX) and flow cytometry

Summary

Background

Prostaglandin (PG) D2 is substantially involved in allergic inflammation and signals via the seven transmembrane (7TM) spanning/G protein-coupled receptors (GPCRs) chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and D-type prostanoid (DP) receptor. While a proinflammatory function of the CRTH2 receptor is well recognized, the role of the DP receptor in allergic inflammation, remains unclear. As it has been shown for many other 7TM/GPCRs, we believe that intermolecular cross-talk occurs between CRTH2 and DP receptors which might be essential for receptor function and regulation

Material and methods

Results

Conclusions