Abstract
Interferons are a group of antiviral cytokines acting as the first line of defense in the antiviral immunity. Here, we describe the antiviral activity of goose type I interferon (IFNα) and type II interferon (IFNγ) against duck plague virus (DPV). Recombinant goose IFNα and IFNγ proteins of approximately 20 kDa and 18 kDa, respectively, were expressed. Following DPV-enhanced green fluorescent protein (EGFP) infection of duck embryo fibroblast cells (DEFs) with IFNα and IFNγ pre-treatment, the number of viral gene copies decreased more than 100-fold, with viral titers dropping approximately 100-fold. Compared to the control, DPV-EGFP cell positivity was decreased by goose IFNα and IFNγ at 36 hpi (3.89%; 0.79%) and 48 hpi (17.05%; 5.58%). In accordance with interferon-stimulated genes being the “workhorse” of IFN activity, the expression of duck myxovirus resistance (Mx) and oligoadenylate synthetases-like (OASL) was significantly upregulated (p < 0.001) by IFN treatment for 24 h. Interestingly, duck cells and goose cells showed a similar trend of increased ISG expression after goose IFNα and IFNγ pretreatment. Another interesting observation is that the positive feedback regulation of type I IFN and type II IFN by goose IFNα and IFNγ was confirmed in waterfowl for the first time. These results suggest that the antiviral activities of goose IFNα and IFNγ can likely be attributed to the potency with which downstream genes are induced by interferon. These findings will contribute to our understanding of the functional significance of the interferon antiviral system in aquatic birds and to the development of interferon-based prophylactic and therapeutic approaches against viral disease.
Highlights
Pattern recognition receptors (PRRs) senses foreign agents in response to pathogen-associated molecular patterns (PAMPs), inducing the production of interferons and proinflammatory cytokines.Interferons, secreted antiviral cytokines that induce a robust immune response, play an important role in both innate and adaptive immunity [1]
Chicken IFNα can suppress the replication of several viruses, such as infectious bronchitis virus (IBV) [3], infectious bursal disease virus (IBDV) [4], and Marek’s disease virus (MDV) [5]
Goose IFNγ has an antiviral effect against goose paramyxovirus (GPMV) in goose fibroblasts and inhibits vesicular stomatitis virus expressing enhanced green fluorescent protein (VSV-Enhanced green fluorescent protein (EGFP)) replication in duck fibroblasts [10]
Summary
Pattern recognition receptors (PRRs) senses foreign agents in response to pathogen-associated molecular patterns (PAMPs), inducing the production of interferons and proinflammatory cytokines. Interferons, secreted antiviral cytokines that induce a robust immune response, play an important role in both innate and adaptive immunity [1]. Pretreatment of Vero cells with chicken IFNγ effectively inhibits vesicular stomatitis virus (VSV) infection [6]. Duck IFNα [7] and IFNγ [8] exhibit a strong inhibitory effect against duck hepatitis B virus (DHBV) in primary duck hepatocytes. Goose IFNγ has an antiviral effect against goose paramyxovirus (GPMV) in goose fibroblasts and inhibits vesicular stomatitis virus expressing enhanced green fluorescent protein (VSV-EGFP) replication in duck fibroblasts [10]. How is the host cellular antiviral state achieved? Interferons bind to their cognate receptors, inducing the expression of interferons and a variety of IFN-stimulated genes (ISGs) [13] such as myxovirus resistance (Mx) protein [14], oligoadenylate synthetases-like (OASL)
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