Cross Species Amplification of Adzukibean Derived Microsatellite Loci and Diversity Analysis in Greengram and Related Vigna Species

Abstract

Greengram or mungbean ( Vigna radiata (L.) Wilczek) is a well known grain legume in Asian countries. Among the different DNA markers, microsatellite or simple sequence repeats (SSRs) are the markers of choice for various genetic studies due to co-dominant nature, loci specificity and high reproducibility. In the present study, a set of thirty-five microsatellite primer pairs derived from adzukibean ( Vigna angularis (Willd.) Ohwi & Ohashi) were used to assess the transferability and tested for their ability to amplify microsatellite loci in greengram and related Vigna species. Of the thirty five microsatellite markers, thirty-two were successfully amplified across the thirty six genotypes and twenty eight were polymorphic. A total of 83 microsatellite alleles were generated with an average of 2.96 alleles per locus. Number of alleles ranged from two to five. Dendrogram formed based on UPGMA, 36 genotypes were grouped into five clusters. Similarly, the neighbour-joining tree developed based on weighted average for dissimilarity matrix grouped 36 genotypes into five groups. The finding suggests that adzukibean derived microsatellite markers are highly informative and could be used to improve the greengram at molecular level.