Cross-Reactivity of Sperm-Binding Proteins from Chicken, Turkey, and Duck Oocytes

Abstract

Binding and penetration of spermatozoa through the perivitelline layer (PL) overlying the hen's ovum have been studied more frequently in the chicken than in other domesticated avian species. Species-specific action of the binding process was tested using an in vitro competition assay in which spermatozoa from the cock, tom, and drake were pretreated with solubilized PL protein (PL-P) from the chicken, turkey, and duck ovum. Spermatozoa were pretreated with PL-P for 20 min at 39 C and co-incubated in vitro with a .5 cm2 section of intact PL from the homologous sperm donor species for an additional 10 min at 39 C.Effectiveness of PL-P pretreatment was assessed quantitatively by the number of spermatozoa bound to the PL, and was expressed as a percentage of the control [minimum essential medium (MEM) pretreated sperm = 100%] binding. Pretreatment of cock spermatozoa with chicken, turkey, or duck PL-P resulted in 21, 40, and 48% binding, respectively. Similarly, pretreatment of tom spermatozoa with PL-P from chicken, turkey, or duck resulted in 45, 51, and 39% binding, and that of drake spermatozoa resulted in 38, 32, and 21% binding, respectively. Incubation of spermatozoa with PL-P from chicken, turkey, and duck ova indicated cross-reactivity and suppression of binding between avian spermatozoa and PL that was not species-specific.