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Abstract
Abstract The cross-reactivity of anti-sulfatide antibody in sera from patients with autoimmune hepatitis was studied. The antibody activity, determined by enzyme-linked immunosorbent assay (ELISA), was reduced in the presence of heparin, heparan sulfate, fucoidan, and dextran sulfate, but not in the presence of keratan sulfate, dermatan sulfate and chondroitin sulfate. The reactivity with sulfatide of serum IgG was bound to a heparin-Sepharose column and a double-stranded DNA-cellulose column, and recovered in the fractions eluted with 1.5 M NaCl. Incubation of patients' sera with heparin and calf thymus DNA reduced the reactivity with sulfatide in 8 and 9, respectively, of 11 patients examined. These findings suggested that anti-sulfatide aantibody was cross-reactive with heparan sulfate, especially heparin, and calf thymus DNA, and that this antibody recognizes certain structures containing repetitive, negatively charged groups as functional epitopes.
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