Abstract
Specific monoclonal antibodies (MAbs) were produced to two hemorrhagic toxins by cell fusion between spleen cells from mice immunized with a hemorrhagic HPLC fraction of Crotalus viridis viridis venom and P3X63Ag8.653 myeloma cells. The MAbs reacted with three different epitopes of high molecular weight hemorrhagic toxins of C. v. viridis venom. A species-specific epitope was detected in a 62 kDa hemorrhagic toxin by one of the MAbs. However, none of the MAbs was able to neutralize the hemorrhagic activity of the toxins. Some epitopes of the hemorrhagic toxins are shared by many related and unrelated species. High molecular weight hemorrhagic toxins ranging from 62 to 68 kDa appeared to be present in the venom of many members of the Crotalidae family. In addition to the high molecular weight toxins, several medium to low molecular weight hemorrhagic toxins were detected in the venoms of C. v. viridis, C. adamanteus, C. atrox and C. h. horridus.
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