Abstract
Cross-linked protein-coated microcrystals (CLPCMCs) of subtilisin Carlsberg (SC), and lipases from Candida rugosa (CRL) and Burkholderia cepacia (BCL) were prepared by cross-linking protein-coated microcrystals (PCMCs). These showed enhanced reaction rates for transesterification in organic solvents of different polarity compared with PCMCs. CLPCMCs of SC were catalytically active up to 80°C in octane and gave 98% conversion in 6 h, while PCMCs gave only 58% conversion in 8 h and conversions did not further increase with time. The Fourier transform infrared spectra showed that the alpha-helical content of CLPCMCs (unlike that of PCMCs) of SC were similar to what is reported for free SC in water.
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