Cross comparison of the IgE binding profiles to recombinant allergens from Suidasia medanensis, Blomia tropicalis and Dermatophagoides farinae using sera from Blomia- and Dermatophagoides-predominant environments

Abstract

Rationale Co-sensitization to Blomia tropicalis and Dermatophagoides spp. are common in populations that are exposed to both mites. We evaluate the use of homologous antigens from a third species, Suidasia medanensis, which is postulated to have allergens that carry fewer and mainly weaker cross-reactive epitopes, as the starting material for the generation of hypoallergens. Methods This study evaluates the IgE binding profiles and cross-reactivity of specific group 1–13 recombinant allergens from Suidasia medanensis, to their counterparts using sera from both Blomia- and Dermatophagoides-predominantly sensitized populations. Results Sensitization to Suidasia medanensis was observed to be significantly weaker than the predominant mites ( Blomia and Dermatophagoides). In both populations, extracts of the predominant mite could totally inhibit IgE binding to Suidasia. Using recombinant allergens, IgE binding to Sui m 1, 3, 6 and 9 were more closely related to that of Blo t 1, 3, 6, and 9. IgE binding to Sui m 2 was however not totally inhibited by Blo t 2 or Der f 2 individually, but in combination reactions to Sui m 2 could be inhibited suggesting the presence unique cross reactive epitopes to both Blo t 2 and Der f 2. IgE binding to Sui d 5 were equivalent to both Blo t 5 and Der f 5, and could be inhibited by both allergens individually. Other recombinant allergens from Suidasia generally exhibited low IgE binding reactions. Conclusions Recombinant allergens from Suidasia medanensis could be used as a starting material for the generation of hypoallergens to dust mites.