Crocin Exerts Neuroprotective Effect in Valproate Exposed Rat Model of Autistic Spectrum Disorder by Ameliorating Behavioural and Biochemical Reversions

Abstract

Autistic spectrum disorder (ASD) is associated with oxidative stress and neuron damage triggered by valproic acid (VPA) in a post-natal rat model of ASD. It was hypothesized that crocin (CRO), may have beneficial effects in ASD due to its antioxidant activities. The study investigated the neuro-behavioural and biochemical modifications of crocin in VPA-induced ASD. Wistar albino rat pups aged 13 days were divided into five groups (06 each). 400 mg/kg VPA subcutaneously on PND 14 successfully induced autism. From PND 14 to 40, crocin was dispensed daily. Different neuro-behavioral markers were examined while the groups were receiving therapy. Animals were sacrificed at the completion of the research for biochemical calculations. Crocin treatment significantly improved behavioral activity and oxidative changes compared to animals treated with the VPA-exposed group. The effects were more pronounced at higher dose of crocin, providing evidence of reversing the valproic acid-induced autistic deficits. Crocin could be a viable option for ASD treatment due to its potential neuronal cytoprotective effects, likely attributed to its antioxidant properties