Abstract
The aim of this study was to explore the possible mechanisms of crocetin affecting rat colitis induced by 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). Rats were treated with crocetin. 5-aminosaliylic acid was used as a positive control. Change of body weight was evaluated. Leukocyte infiltration was detected by myeloperoxidase activity assay. The expression of cyclooxygenase-2 was detected by RT-PCR and Western blot. Inflammatory cytokines were determined by RT-PCR. Local concentration of prostaglandin E 2 in colon mucosa was determined by ELISA. Crocetin decreased the macroscopic scores and myeloperoxidase activity. Crocetin also reduced the expression of COX-2 and inflammation cytokines. In addition, treatment with crocetin increased the PGE 2 level. Moreover, the effects of crocetin were almost comparable with that of positive control drug. Crocetin has therapeutic effects on TNBS-induced colitis; the mechanisms seem to be related to COX-2 inhibition and PGE 2 improvement.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
