Abstract
SummaryImages obviously are one of the most important category among the available data in everyday clinical ophthalmology and the visualization of different layers of retina. In experimental eye research it also play pivotal role in understanding of different biological processes of retina. This talk will focus on image capturing and analysis of mouse retina with different genetic background. In our project, we have focused on retinal pigmented epithelium (RPE) obtained from different degenerative mouse models mimicking cellular changes observed in Age‐related Macular Degeneration (AMD). The 2D and 3D of RPE images were acquired through different types of microscopy. We deliver results on modern methods of absolute nucleic acid or protein quantification (q‐PCR, WB, ELISA), but highlight the importance of imaging of the bio‐molecular processes by a range of powerful imaging weapons (stereo/light/confocal/EM/microMRI). We have carried out do novo, in and ex vivo analyzis at micro and ultrastructural level to describe the molecular fingerprints of mouse RPE features in AMD.
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