Abstract
The effect of freezing rate, freezing temperature, freezing time and the cryoprotective capabilities of a number of additives on the carboxyfluorescein (CF) retention and physical stability (resistance against aggregation and fusion) of liposomes after a freezing/ thawing cycle was investigated. Negatively charged multilamellar vesicles consisting of hydrogenated soybean lecithin and DCP (10/1) were used. No aggregation or fusion and over 90% CF retention was found after freezing at −25°C (freezing rate 7°C min −1) for 20 min in the presence of various cryoprotectants; other freezing conditions proved to induce a substantial CF loss or aggregation and fusion.
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