Abstract
The ion selectivity of a channel can be quantified in several ways by using differentexperimental protocols. A wide, mesoscopic channel, the OmpF porin of the outermembrane of E. coli, serves as a case study for comparing and analysing several measuresof the channel cation–anion permeability in chlorides of alkali metals (LiCl, NaCl, KCl,CsCl). We show how different insights can be gained and integrated to rationalize theglobal image of channel selectivity. To this end, reversal potential, channel conductance andbi-ionic potential (two different salts with a common anion on each side of thechannel but with the same concentration) experiments are discussed in light of anelectrodiffusion model based on the Poisson–Nernst–Planck formalism. Measurements andcalculations based on the atomic crystal structure of the channel show that eachprotocol displays a particular balance between the different sources of selectivity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
