Abstract
CRISPR system is a powerful defense mechanism in bacteria and archaea to provide immunity against viruses. Recently, this process found a new application in intended targeting of the genomes. CRISPR-mediated genome editing is performed by two main components namely single guide RNA and Cas9 protein. Despite the enormous data generated in this area, there is a dearth of high throughput resource. Therefore, we have developed CrisprGE, a central hub of CRISPR/Cas-based genome editing. Presently, this database holds a total of 4680 entries of 223 unique genes from 32 model and other organisms. It encompasses information about the organism, gene, target gene sequences, genetic modification, modifications length, genome editing efficiency, cell line, assay, etc. This depository is developed using the open source LAMP (Linux Apache MYSQL PHP) server. User-friendly browsing, searching facility is integrated for easy data retrieval. It also includes useful tools like BLAST CrisprGE, BLAST NTdb and CRISPR Mapper. Considering potential utilities of CRISPR in the vast area of biology and therapeutics, we foresee this platform as an assistance to accelerate research in the burgeoning field of genome engineering.Database URL: http://crdd.osdd.net/servers/crisprge/.
Highlights
Genome editing is a method to target any desired sequence in the genome
CrisprGE is a dedicated repository having total of 4680 genes edited by CRISPR/Cas approach
CRISPR/Cas-based genome editing has been extensively explored since invention of Single guide RNA (sgRNA)
Summary
This technique has earned significant achievements in the area of therapeutics or gene therapy with the help of artificially designed nucleases [1]. In this method, a sequence-specific DNA-binding domain is fused to a nuclease domain that cuts DNA at intended site with high efficiency but in non-sequence specific manner [2]. A new class of nucleases, known as, Clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins (CRISPR/ Cas) has emerged in recent times.
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