Year-end Sale % OFF 
Abstract
BackgroundLeaves, which are the most important organs of plants, can not only fix carbon sources through photosynthesis, but also absorb nutrients through transpiration. Leaf development directly determines the growth, flowering and fruiting of plants. There are many factors that affect leaf development, such as the growth environment, gene expression, and hormone synthesis. In this study, tomatoes were used to study the role of the transcription factor Solanum lycopersicum salt-related MYB1-like (SlSRM1-like) in the development of tomato leaves.ResultsLoss-of-function of the SlSRM1-like gene mediated by clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) resulted in abnormal tomato leaf morphology, including thinner leaves, wrinkled edges, raised veins, disordered edge veins, and left and right asymmetry. An analysis of the transcription levels of genes related to leaf development revealed that the expression of these genes was significantly altered in the SlSRM1-like mutants (SlSRM1-like-Ms). Moreover, the SlSRM1-like gene was expressed at higher transcription levels in young tissues than in old tissues, and its expression was also induced in response to auxin. In addition, the transcription levels of genes related to the auxin pathway, which regulates tomato growth and development, were severely affected in the SlSRM1-like-Ms. Therefore, it is hypothesized that the SlSRM1-like gene functions in the regulation of tomato leaf development through the auxin-related pathway.ConclusionsIn this study, we successfully knocked out the SlSRM1-like gene in the tomato variety Ailsa Craig using CRISPR technology and found that knockout of the SlSRM1-like gene resulted in abnormal development of tomato leaves. Further research indicated that SlSRM1-like regulated tomato leaf development through auxin-related pathways. The results provide an important reference for the functional study of other SRM1-like genes in plants and provide new insights into the regulation of leaf development in tomato and other plants.
Highlights
Leaves, which are the most important organs of plants, can fix carbon sources through photosynthesis, and absorb nutrients through transpiration
SlSRM1‐like is a MYB‐related transcription factor located in the nucleus When we analyzed the transcriptome data of leaves from different cultivated tomato plants, we noticed the Solyc04g008870.2.1 gene by chance
We compared its sequence with others via the Basic Local Alignment Search Tool (BLAST) and found that this gene was predicted to be Salt-Related MYB1 (SRM1)-like, which is a member of the MYBrelated transcription factor family
Summary
Leaves, which are the most important organs of plants, can fix carbon sources through photosynthesis, and absorb nutrients through transpiration. There are many factors that affect leaf development, such as the growth environment, gene expression, and hormone synthesis. There are many factors that affect leaf development, generally through regulation of the plant’s own gene expression and growth environment [14, 15]. Mutants of rice (Oryza sativa) have narrowed leaves, which is directly related to a decrease in auxin content in the leaves [24] Another rice mutant, the narrow leaf (nal21) mutant, exhibits obvious changes in leaf width, leaf length, leaf veins, and the size and number of epidermal cells, which is caused by an abnormal auxin response because of the lack of function of the ribosomal small subunit protein RPS3A encoded by NAL21 [25]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
