CRISPR/Cas9-mediated gene editing of GmJAGGED1 increased yield in the low-latitude soybean variety Huachun 6.

Abstract

The number of seeds per pod (NSPP) is one of the critical components that affect soybean yield (Liu et al., 2020). Ln, which encodes GmJAGGED1 (GmJAG1), is a major locus regulating NSPP and leaflet shape in soybean (Fang et al., 2013; Jeong et al., 2012). A single base-pair substitution of Ln locus from guanine (G) to cytosine (C) led to an amino acid change in the conserved EAR motif of GmJAG1, resulting in the loss-of-function of GmJAG1 and ln (Fang et al., 2013; Jeong et al., 2012). ln can significantly increase NSPP (Fang et al., 2013) and has been widely used in soybean breeding.