Abstract
Clustered Regularly Interspaced Short Palindromic Repeats is a new and advance gene editing tool using specific nuclease enzyme for specific cleavage. It is the most efficient technique, commonly used for many purposes like gene therapy, production of desired plants and transgenic animals. But it has some limitations like off-target issue and this issue can be minimized by the production of specific sequence of guide RNA. In the future, it can be used for the treatment of many human genetic diseases
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