Abstract
The small crucifer Cardamine hirsuta bears complex leaves divided into leaflets. This is in contrast to its relative, the reference plant Arabidopsis thaliana, which has simple leaves. Comparative studies between these species provide attractive opportunities to study the diversification of form. Here, we report on the implementation of the CRISPR/Cas9 genome editing methodology in C. hirsuta and with it the generation of novel alleles in the RCO gene, which was previously shown to play a major role in the diversification of form between the two species. Thus, genome editing can now be deployed in C. hirsuta, thereby increasing its versatility as a model system to study gene function and evolution.
Highlights
Over the past few years Cardamine hirsuta, a small crucifer related to the reference plant Arabidopsis thaliana, has emerged as a powerful model system for comparative developmental studies in plants
Both species belong to Lineage I of the Brassicaceae, and parallel genetic studies have provided a powerful platform to identify the molecular causes of trait diversity between these two species and for understanding the morphogenetic basis [1,2,3,4,5,6]
As genome editing has opened considerable new potential for efforts to connect genotype to phenotype through development and evolution [15], it became important to set up a CRISPR/Cas9 genome editing in this system
Summary
Over the past few years Cardamine hirsuta, a small crucifer related to the reference plant Arabidopsis thaliana, has emerged as a powerful model system for comparative developmental studies in plants.Both species belong to Lineage I of the Brassicaceae, and parallel genetic studies have provided a powerful platform to identify the molecular causes of trait diversity between these two species and for understanding the morphogenetic basis [1,2,3,4,5,6]. Studies of leaf development in particular have led to major advances in identifying both causal genes and regulatory sequences for the diversification of form and the broader logic through which morphological evolution proceeds [2,7,8,9,10,11]. These efforts were underpinned by the development of essential genetic resources, including genetic and cytogenetic maps, a reference genome sequence, recombinant inbred populations, and of a stable gene transformation protocol by floral dipping [12,13,14].
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