CRF1/CRF2 and MC3/MC4 Receptors Affect Glutamate- Induced Food Intake in Neonatal Meat-Type Chicken

F Ahmadi,M Zendehdel,N Panahi,V Babapour

doi:10.1590/1806-9061-2018-0821

F Ahmadi, M Zendehdel + Show 2 more

Open Access

https://doi.org/10.1590/1806-9061-2018-0821

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Abstract

ABSTRACT Central glutamate, melanocortin and corticotropin systems have mediatory role on several physiologic functions in the brain, but their interactions on appetite regulation are not fully elicited. So, the aim of the current study was to determine interaction of the glutamate with melanocortin and corticotropin systems on food intake in 3-h food-deprived (FD3) neonatal meat-type chicken. In experiment 1, chicken intracerebroventricular (ICV) injected (A) phosphate-buffered saline (PBS), (B) glutamate (75 nmol), (C) glutamate (150 nmol) and (D) glutamate (300 nmol). In experiment 2, (A) PBS, (B) astressin-B (CRF1/CRF2 receptors antagonist, 30 µg), (C) glutamate (300 nmol) and (D) astressin-B+glutamate were ICV injected. Experiments 3-5 were similar to experiment 2, except birds were injected with astressin2-B (CRF2 receptor antagonist, 30 µg), SHU9119 (MC3/MC4 receptor antagonist, 0.5 nmol) and MCL0020 (MC4 receptor antagonist, 0.5 nmol) instead of the astressin-B. In experiment 6, the injections were (A) PBS, (B) MTII (MC3/MC4 receptor agonist, 2.5ng), (C) glutamate (75nmol) and (D) MTII+glutamate. Then, cumulative feed intake was recorded at 30, 60 and 120 minutes after injection. According to the results, dose dependent hypophagia observed by ICV injection of the glutamate (75, 150 and 300nmol) compared to control group in neonatal broiler chicken (p<0.05). Co-injection of the astressin-B+glutamate and astressin2-B+glutamate decreased glutamate-induced hypophagia in neonatal broiler chicken (p<0.05). Co-injection of the glutamate+MC3/MC4 receptors antagonist decreased hypophagic effect of the glutamate (p<0.05). These results suggested hypophagic effect of the glutamate mediates via CRF1/CRF2 and MC3/MC4 receptors in chickens.

Highlights

Feed intake, satiety and energy expenditure regulates via diverse signals from central and peripheral tissues (Hassanpour et al, 2015; Zendehdel et al, 2017)
CRF1, CRF2, MC3 and MC4 receptors on cumulative feed decreased feed intake compared to the control group eRBCA-2019-0821
In experiment 6, sole ICV injection of the MTII (2.5 ng) or glutamate (75 nmol) had no effect on feeding behavior in FD3 neonatal broilers compared to the control group (P >0.05)

Summary

Introduction

Satiety and energy expenditure regulates via diverse signals from central and peripheral tissues (Hassanpour et al, 2015; Zendehdel et al, 2017). Neurotransmitters interact by a wide distributed neurological network on feed intake regulation in the central nervous system (CNS) (D’Addario et al, 2014). The melanocortin system is one of the central neurotransmitter systems and to date its five subtypes (MC1R-MC5R) have been identified (Alvaro et al, 2003). It has prominent role in several physiologic functions e.g. grooming, thermoregulation, learning and energy balance regulation (Schneeberger et al, 2014). In the brain of the avian Melanocortin receptors have been identified

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