Abstract
In this paper, the application of a non-ionic detergent Cremophor EL for monomerization of chlorophyll a in an aqueous medium is studied. The spectrophotometric properties of chlorophyll a encapsulated into the Cremophor EL nano-emulsion system were characterized by electronic absorption, steady-state and time-resolved fluorescence as well as circular dichroism spectroscopy. The results have shown that chlorophyll a dissolves more efficiently in the aqueous medium containing low-level Cremophor (5 wt%) than at an ethanolic solution even in the concentration of 10−4 M. The molecular organization of the chlorophyll a in the Cremophor EL nano-micelles was also investigated by means of Raman spectroscopy. The spectral changes in the frequency of the C=O stretching group were used to distinguish the aggregation state of chlorophyll. It was revealed that chlorophyll a exists dominantly in the monomeric form in the Cremophor EL aqueous solution. The promising aspect of the use of Cremophor EL nano-emulsion as a delivery system is to maintain stable chlorophyll monomer in an aqueous medium. It would open the potential for a new, practical application of chlorophyll a in medicine, as a dietary supplement or studies on molecular organization of chlorophyll a in the well-defined artificial system.
Highlights
There are few biomolecules related to their chemical structure investigation that have been mentioned by the laureates in the Nobel award lectures [1,2,3]
The analysis has shown double (100% Cremophor EL (CrEL)) or triple-exponential decay kinetics (5 wt% CrEL nano-emulsion and 96% EtOH) indicating the presence of different excited molecular species in the samples
Chl a was encapsulated into the CrEL nano-emulsion system
Summary
There are few biomolecules related to their chemical structure investigation that have been mentioned by the laureates in the Nobel award lectures [1,2,3]. Chlorophylls (chls), which are largely involved in the biological process such as photosynthesis, is often referred to be “the pigments of life” [4]. Chlorophylls (chls) are the most abundant class of biomolecules present in the world. As evidenced by a number studies, they are much more than just green pigments found in the photosynthetic organisms. Their extensive use in the pharmaceutical and food industries was reported [5,6]. Biomedical applications illustrate the use of chlorophyll as a photosensitizer in the photodynamic therapy (PDT), Biomolecules 2019, 9, 881; doi:10.3390/biom9120881 www.mdpi.com/journal/biomolecules
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