Abstract
espanolEl objetivo de la presente investigacion fue evaluar el crecimiento y el contenido de pigmentos de un nuevo aislado de Spirulina subsalsa cultivada en agua mar a diferentes salinidades y concentraciones de nitrogeno. La nueva cepa de S. subsalsa se aislo a partir de muestras de agua procedentes del embalse de Clavellino, estado Sucre, Venezuela, y fue identificada haciendo uso de la clave taxonomica propuesta por Aguiar (2013). El medio de cultivo ensayado fue el f/2, modificando las concentraciones de nitrato (14,5; 29 y 58 mmol/L) y cloruro de sodio (0, 9 y 18 ‰ por adicion de agua de mar). Los cultivos se realizaron por triplicado, de forma discontinua, bajo condiciones de medio ambiente controlado (T: 30±1 oC; iluminacion: 3.000 lux; agitacion manual, fotoperiodo 12:12), durante 21 dias. Los resultados obtenidos evidenciaron que la salinidad de 9 ‰ y la concentracion de nitrato de 14 mmol/L fueron los parametros que propiciaron los mayores contenidos de biomasa; mientras que la clorofila a y la ficocianina mostraron mayores valores en la misma concentracion de nitrogeno, pero a 0 ‰. Estos hallazgos indican que la salinidad y la concentracion de nitrogeno afectan el crecimiento y los pigmentos del nuevo aislado de S. subsalsa y ademas sugieren que esta cepa posee potencial para su aprovechamiento biotecnologico con miras a obtener metabolitos valiosos en las industrias alimenticias y farmacologicas. EnglishThe aim of this research was to evaluate the growth and content of pigments of a new isolate of Spirulina subsalsa grown in seawater at different salinities and nitrogen concentrations. The new S. subsalsa strain was isolated from water samples from the Clavellino reservoir, Sucre state, Venezuela, and was identified using the taxonomic key proposed by Aguiar (2013). The culture medium tested was f / 2 Guillard, modifying the concentrations of nitrate (14.5; 29, 59 mmol/L) and sodium chloride (0, 9 and 18 ‰ by the addition of seawater). The cultures were carried out in triplicate, discontinuously, under conditions of controlled environment (T: 30±1 °C, illumination: 3,000 lux, manual agitation, photoperiod 12:12), for 21 days. The results obtained showed that the salinity of 9 ‰ and the nitrate concentration of 14 mmol/L were the parameters that propitiated the highest biomass; while chlorophyll a and phycocyanin showed higher values at the same nitrogen concentration at 0 ‰. These findings indicate that salinity and nitrogen concentration affect the growth and pigments of the new isolate of S. subsalsa and suggest that this strain has potential for its biotechnological exploitation in order to obtain valuable metabolites for food and pharmacological industries.
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