Abstract
A human chromosome or its subchromosomal fragment (SCF) derived from normal fibroblasts was introduced into mouse embryonic stem (ES) cells via microcell-mediated chromosome transfer (MMCT) and viable chimaeric mice were produced from them. Serum antibodies with each human Ig were detected and various V segments were identified in human Ig H, κ and λ transcripts. Upon immunization of the chimaeras with HSA, HSA-specific antibodies with hγ hκ and λ were detected in the sera. A HSA-specific human antibody with hμ producing hybridoma was obtained from fusion of murine myelomas with spleen cells from the chimaeric mouse.KeywordsEmbryonic StemHuman Serum AlbuminHuman ChromosomeMouse Embryonic Stem CellHuman AntibodyThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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