Creation of Antibody Immunoassay Test Systems for Indication of Enteropathogenic Yersinia

V Kuznetsova,Sergey Ivaschenko,N.A Shyurova,O.V Tkachenko

doi:10.1051/bioconf/20224303042

Abstract

3 immunoassay test systems based on antibodies obtained as a result of immunization of rabbits with one of the antigens: disintegrated membranes (DM) Y. enterocolitica, DM Y. pseudotuberculosis and lipopolysaccharide Y. pseudotuberculosis were created. The best diagnostic properties were possessed by a test system based on antibodies to Y. pseudotuberculosis DM. It showed high titers of antibodies to various serovars of Y. pseudotuberculosis (1:25600-1:51200), incl. to microbe strains isolated from farm animals, and low titers to Y. enterocolitica (1:200). In the accumulation media with pig excrements seeded with Yersinia, the experimental test system detected Y. pseudotuberculosis already on the 3rd day of “cold enrichment” provided that 50 or more Yersinia cells per ml were inoculated into the accumulation medium together with excrements.

Highlights

The pseudotuberculosis microbe is secreted from pigs, cattle, sheep, goats, deer, monkeys, migratory birds [1, 2, 3, 4, 5]
3 enzyme-linked immunosorbent assay systems were created based on antibodies to Y. enterocolitica disintegrated membranes (DM) (Y. е.DM), Y. pseudotuberculosis DM
The sensitivity and specificity of the created test systems were studied in reaction with whole cells of Y. pseudotuberculosis and Y. enterocolitica

Summary

Introduction

The pseudotuberculosis microbe is secreted from pigs, cattle, sheep, goats, deer, monkeys, migratory birds [1, 2, 3, 4, 5]. For the eastern part of Europe, circulation in pigs is characteristic: O:3 serovars of the pseudotuberculosis microbe (100 % of the isolated cultures), for the central part – O:3 (60 %), O:1 (20 %), O:2 (20 %), for the southern part – O:1 (60 %), O:3 (20 %), for England – O:3 (34 %), O:1 (26 %), O:2 (24 %) [6, 7, 8]. To get rid of them it is possible using "cold accumulation" of Yersinia on "poor" liquid nutrient media, followed by sowing on dense combined nutrient media with selective and differential diagnostic properties. When examining excrements, the diagnostic efficiency of Endo medium is 11 %, McConkey medium – 33 %, Serov medium – 42 %, SBTS – 81 % [9, 10]

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Conclusion