Abstract
We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P protein-deleted virus (P−) grew very inefficiently unless both of the viral accessory proteins (V and C) were also expressed. Growth of the virus was restricted to the P-expressing cell line. The P− virus grew more slowly than the parental virus and expressed much less viral protein in infected cells. The technique could be used to create virus-like particles for use as a vaccine or as antigen in immunological or serological assays.
Highlights
We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein
The P gene gives rise to two non-structural or accessory proteins, V and C, which play a variety of roles in modulating host immune responses and the dynamics of virus replication (e.g. Baron & Barrett, 2000; Nakatsu et al, 2008; Nanda & Baron, 2006; Ohno et al, 2004; Palosaari et al, 2003; Parks et al, 2006; Takeuchi et al, 2003; Tober et al, 1998), but are not essential proteins in the virus life cycle, since recombinant morbilliviruses lacking either or both proteins have been made which grow in cell culture
The P protein was selected as the viral protein to provide in trans as it acts as a subunit of the polymerase, as well as working with the N protein in encapsidation of the genome, and is completely indispensable in the virus life cycle
Summary
We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P” virus grew more slowly than the parental virus and expressed much less viral protein in infected cells.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
