Creation and micropropagation of polyploids in Cymbidium hybridum

Abstract

The protocorm-like bodies of a di-triploid Cymbidium hybridum were subjected for polyploid creation by the use of colchicine treatment. Diploid and triploid plants were obtained from both the control and colchicine treatments, and tetraploid and hexaploid plants were identified in the regenerated populations of colchicine treatment. Micropropagation charateristic of C. hybridum was obviously changed after chromosome doubling. The proliferation rate of diploid and tetraploid was significantly higher than that of triploid. The bud differentiation rate of diploid was the highest, followed by the tetraploid, and that of triploid were the lowest. The root differentiation rate of diploid, triploid and tetraploid was all 100%; however, the mean number, length and diameter of the root were significantly different. The suitable medium for micropropagation of diploid, triploid and tetraploid was also different.