Abstract
Atopic dermatitis (AD) has a drastic impact on human health owing to complex skin, gut microbiota, and immune responses. Some lactic acid bacteria (LAB) are effective in ameliorating AD; however, the alleviative effects of dairy products derived from these LAB remain unclear. In this study, the efficacies of Lactococcus chungangensis CAU 28 (CAU 28) cream cheese and L. chungangensis CAU 28 dry cells were evaluated for treating AD in an AD mouse model. Overall, CAU 28 cream cheese administration was more effective against AD than L. chungangensis CAU 28 dry cells. Faeces from CAU 28 cream cheese-administered mice had increased short chain fatty acid, butyrate, acetate, and lactic acid levels, as well as butyrate-producing bacteria, including Akkermansia, Bacteroides, Lactobacillus, and Ruminococcus. Furthermore, oral CAU 28 cream cheese administration resulted in regulatory T cell (Treg)-mediated suppression of T helper type 2 (Th2) immune responses in serum and mRNA expression levels in the ileum. Oral CAU 28 cream cheese further reduced IgE levels, in addition to eosinophil and mast cell numbers. Therefore, CAU 28 cream cheese administration induced a coordinated immune response involving short-chain fatty acids and gut microbiota, indicating its potential for use as a supplement for AD mitigation.
Highlights
Atopic dermatitis (AD) has a drastic impact on human health owing to complex skin, gut microbiota, and immune responses
Five-weekold female BALB/c mice (n = 50) were randomly assigned to five groups (n = 10/group) as follows: (1) negative control group, which included mice that were not subjected to ovalbumin (OVA) sensitization and orally administered with phosphate-buffered saline (PBS); (2) positive control group, sensitized with OVA and orally administered with PBS; (3) bepotastine besilate (BB) group, sensitized with OVA and orally administered with BB; (4) L. chungangensis CAU 28 (CAU 28) group, sensitized with OVA and orally administered with freeze-dried L. chungangensis CAU 28; (5) L. chungangensis CAU 28 (CAU 28) cream cheese group, sensitized with OVA and orally administered with cream cheese prepared using L. chungangensis CAU 28
The most frequently used strain in these studies was L. rhamnosus GG, and a number of studies have suggested that this strain is beneficial in preventing the onset AD, either alone or as part of a probiotic mixture[28,29]; several other studies reported that the L. rhamnosus GG strain, either alone or as part of mixed cultures, has limited effects[24,25] or confers no significant benefit[30] in the treatment of AD
Summary
Atopic dermatitis (AD) has a drastic impact on human health owing to complex skin, gut microbiota, and immune responses. Faeces from CAU 28 cream cheese-administered mice had increased short chain fatty acid, butyrate, acetate, and lactic acid levels, as well as butyrate-producing bacteria, including Akkermansia, Bacteroides, Lactobacillus, and Ruminococcus. CAU 28 cream cheese administration induced a coordinated immune response involving short-chain fatty acids and gut microbiota, indicating its potential for use as a supplement for AD mitigation. The consumption of fermented dairy products incorporating beneficial bacteria modifies the gut microbiota toward an increase in production of butyrate (a short-chain fatty acid [SCFA] metabolite produced by gut microbiota) relative to that induced by chemically acidified milk[4]. Among LAB, Lactococcus spp. are used as starter cultures for the production of cheese and fermented milk-based products; their probiotic function has frequently been underestimated because of an assumption that they cannot survive in the gastrointestinal tract[11]. This strain was previously examined by transcriptomic analysis for the presence of functional genes, including those encoding cystathionine β-lyase (MetC), O-acetylserine sulfhydrylase (CysK), www.nature.com/scientificreports/
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
