야생산사(Crataegus pinnatifida BUNGE)씨 생리활성물질의 산화억제 효과

Abstract

본 연구에서는 야생산사씨의 항산화 활성을 알아보고자 70% methanol, 70% ethanol, chloroform:methanol (CM, 2:1, v / v), n-butanol, ethyl acetate (EA) 용매로 추출하여, 시료 농도 0.2 mg/ml, 0.5 mg/ml, 0.8 mg/ml에서 측정 비교 하였다. DPPH, reducing power, FRAP 및 ABTS radical 소거활성은 70% methanol, 70% ethanol, EA, CM, n-butanol 추출물 순으로 높게 측정되어 모두 같은 경향을 나타내었다. 특히, DPPH에서 70% methanol 추출물은 18.22%~65.42%, 70% ethanol 추출물은 15.97%~60.25%의 우수한 radical 소거 활성을 보였다. NO radical 소거 활성은 n-butanol, EA, CM, 70% methanol, 70% ethanol 추출물 순으로 나타났으며, <TEX>$OH^-$</TEX> radical 소거 활성은 n-butanol, 70% methanol, 70% ethanol, CM, EA 추출물 순으로 측정되었다. 모든 용매 추출물은 대조군으로 사용한 표준물질에 비해서는 유의적으로 항산화 활성이 낮은 것으로 나타났다. 산사씨 용매 추출물 중 70% methanol 및 70% ethanol 추출물의 항산화 활성이 대부분 높은 경향이었다. 따라서 산사씨는 우수한 천연 항산화제로 활용이 가능할 것으로 사료된다. Haw (Crataegus pinnatifida <TEX>$B_{UNGE}$</TEX>) is a medicinal plant commonly used in Korea due to its beneficial health effects. This study was performed to investigate the antioxidative activity of bioactive components from haw seed. Freeze-dried seed was processed with various solvents such as 70% methanol, 70% ethanol, a mixture of chloroform and methanol (2:1, v / v), n-butanol and ethyl acetate (EA). DPPH, reducing power, ferric reducing antioxidant power (FRAP), and ABTS radical scavenging activity decreased in this order: 70% methanol, 70% ethanol, EA, CM, n-butanol extracts. NO radical scavenging activity was high in the n-butanol extract (36.73% in 0.8 mg/ml concentration) and low in the 70% ethanol extract (24.96% in 0.8 mg/ml concentration). <TEX>$OH^-$</TEX> radical scavenging activity of n-butanol extract (40.42% in 0.8 mg/ml concentration) was higher than the other extracts, but EA extract was the lowest (13.39%) at the same concentration. The results show that each extract contained various antioxidative substances and it seemed that different influence at each antioxidative activity.