Abstract
The objective of this study was to investigate if cryopreservation of teeth for long-term storage leads to cracks in enamel and dentin. Three teeth, which were extracted for orthodontic reasons, were cryopreserved in liquid nitrogen (temperature -196°C) and thawed according to standard protocols after 4 months. Micro computed tomography using synchrotron radiation was performed to detect cracks in the tooth hard tissues. Cracks were found in the enamel of all teeth, which are associated with forceps application during extraction. Cracks with a width larger than 0.8 μm were not identified in dentin and cementum. Although cryopreservation of teeth according to the standard protocol does not generate cracks more than 0.8 μm wide, the use of forceps can result in prominent cracks.
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