Abstract
Cracking the (tubulin) code of mitosis.
Highlights
Mitosis in high eukaryotes is typically initiated by nuclear envelope breakdown (NEB), allowing the dynamic interaction between microtubules and condensed chromosomes
Unlike tubulin acetylation, which takes place on the luminal side of microtubules, tubulin detyrosination occurs at the very end of α-tubulin c-terminal, and is located at the outer surface of the microtubule lattice, where it is more likely to interact with motor proteins
We developed two strategies to decrease the levels of microtubule detyrosination in cells
Summary
Mitosis in high eukaryotes is typically initiated by nuclear envelope breakdown (NEB), allowing the dynamic interaction between microtubules and condensed chromosomes. We addressed this question by investigating the impact of post-translational modifications (PTMs) of tubulin on CENP-E activity and function. This was based on the hypothesis that the activity of motor proteins is not affected exclusively via its molecular regulation (e.g. by phosphorylation), and might be achieved indirectly, by modifying the tracks in which they move on (e.g. by post-translational modifications of microtubules).
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