Abstract
In the previous study, we reported purification and characterization of cytosolic protein-tyrosine kinase, CPTK71, from bovine platelets (Nakamura, S., Yanagi, S. and Yamamura, H. (1988) Eur. J. Biochem. 174, 471-477). In the present study, we have investigated the relationship between CPTK71 and p72 syk which was purified from cytosolic fraction of porcine spleen and sequenced from porcine cDNA library. The elution patterns of CPTK71 activity in each purification step closely corresponded with the pattern of immunoreactivity with p72 syk . Immunoprecipitation study revealed that specific antibodies against p72 syk precipitated the activity of both phosphorylating [Val 5]angiotensin II and autophosphorylation of highly purified CPTK71. In addition, the same result was obtained using another anti-p72 syk antibody which recognized different amino acid sequence of 72 syk . From these results, we conclude that CPTK71 is identical or closely related to p72 syk .
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