Cpne7 is a signaling molecule from preameloblasts and regulates the differentiation of mesenchymal cells into odontoblasts

Abstract

Event Abstract Back to Event Cpne7 is a signaling molecule from preameloblasts and regulates the differentiation of mesenchymal cells into odontoblasts Joo-Cheol Park1, Han-Wool Choung1 and Hyun-Sook Bae2 1 School of Dentistry, Seoul National University, Department of Oral Histology-Developmental Biology, Korea 2 Namseoul University, Department of Dental Hygiene, Korea Stem cell biology has emerged as one of the fundamental underpinnings for regenerative medicine. To achieve a clinical success with stem cell based therapy, it is important to understand the molecular and cellular basis of stem cells. Tooth development involves sequential interactions between dental epithelial and mesenchymal cells. Our previous studies demonstrated that preameloblast-conditioned medium (PA-CM) induces the odontogenic differentiation of human dental pulp cells (hDPCs) and promotes dentin formation. PA-CM can induce vascularized pulp regeneration of hDPSCs with physiological structures in an empty root canal space. The protein Cpne7 was identified in PA-CM by liquid chromatography-tandem mass spectrometry and suggested as a candidate signaling molecule regulating odontoblast differentiation. We investigated biological function and mechanisms of Cpne7 in regulation of odontoblast differentiation. Cpne7 was expressed in preameloblasts and secreted to the extracellular matrix during ameloblast differentiation. After secretion, Cpne7 protein was translocated to differentiating odontoblasts. In differentiating odontoblasts, Cpne7 overexpression or treatment with recombinant Cpne7 significantly promoted the expression of Dspp in vitro. In contrast, siRNA-mediated inactivation of Cpne7 suppressed Dspp expression. In in vivo transplantation experiments, Cpne7 induced odontoblast differentiation and promoted formation of dentin/pulp-like tissue in dental mesenchymal cells, such as hDPCs. Moreover, Cpne7 induced differentiation into odontoblasts of undifferentiated mesenchymal C3H10T1/2 cells in vitro and of non-dental mesenchymal cells, such as human bone marrow mesenchymal stem cells, with formation of dentin-like mineralized tissues including the structure of dentinal tubules in vivo. Mechanistically, Cpne7 interacted with Nucleolin and modulated odontoblast differentiation via the control of Dspp expression. These results suggest Cpne7 is a diffusible signaling molecule that is secreted by preameloblasts and regulates the differentiation of mesenchymal cells of dental or non-dental origin into odontoblasts. Therefore, regulation of Cpne7 expression in mesenchymal stem cells could be a novel therapeutic approach for the treatment of diseased dentin-pulp complex. Keywords: Cell Differentiation, stem cell, growth factor, Tissue Regeneration Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Park J, Choung H and Bae H (2016). Cpne7 is a signaling molecule from preameloblasts and regulates the differentiation of mesenchymal cells into odontoblasts. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02430 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Joo-Cheol Park Han-Wool Choung Hyun-Sook Bae Google Joo-Cheol Park Han-Wool Choung Hyun-Sook Bae Google Scholar Joo-Cheol Park Han-Wool Choung Hyun-Sook Bae PubMed Joo-Cheol Park Han-Wool Choung Hyun-Sook Bae Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.