CpG Oligodeoxynucleotide/cationic peptide nanorings as immunotherapeutic agents

Abstract

Event Abstract Back to Event CpG Oligodeoxynucleotide/cationic peptide nanorings as immunotherapeutic agents Bilgi Gungor1*, Fuat C. Yagcı2, Ihsan Gursel2 and Mayda Gursel1 1 Middle East Technical University, Biological Sciences, Turkey 2 Bilkent University, Department of Molecular Biology and Genetics, Turkey Synthetic CpG containing oligodeoxynucleotides (ODNs) mimic the immunostimulatory activity of bacterial DNA and induce a strong pro-inflammatory response via Toll-like receptor 9 (TLR9) signaling. To date, most of the human clinical trials involved one type of CpG ODN (known as K-type or B-type) whereas the therapeutic potential of the G-quadruplex forming type I interferon inducing D-type ODN (or A-type) remained unexplored due to product aggregation that complicates the pharmaceutical manufacturing of this ODN type. Herein we show that a short K-type ODN and the HIV-derived cationic peptide Tat(47-57) condense to form monodisperse nuclease resistant nanorings that replicate the high type I interferon stimulating activity of D-type ODN in human blood. The nanorings provide nuclease resistance, enhance the cellular uptake and alter the subcellular distribution of the associated CpG ODN. Consistent with their IFNa inducing activity from plasmacytoid dendritic cells, the nanorings localize to early endosomes. In conclusion, these immunostimulatory nanorings are effective D-ODN surrogates and could prove to be of value as anti-viral or anti-cancer agents and vaccine adjuvants in the clinic. Acknowledgements This work was supported by a TUBITAK grant (111S151) Keywords: CpG ODN, TLR 9, IFNa, Tat peptide, Nanoring Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Gungor B, Yagcı FC, Gursel I and Gursel M (2013). CpG Oligodeoxynucleotide/cationic peptide nanorings as immunotherapeutic agents. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00171 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Bilgi Gungor, Middle East Technical University, Biological Sciences, Ankara, 06800, Turkey, bilgigungor@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Bilgi Gungor Fuat C Yagcı Ihsan Gursel Mayda Gursel Google Bilgi Gungor Fuat C Yagcı Ihsan Gursel Mayda Gursel Google Scholar Bilgi Gungor Fuat C Yagcı Ihsan Gursel Mayda Gursel PubMed Bilgi Gungor Fuat C Yagcı Ihsan Gursel Mayda Gursel Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.