Abstract
Abstract Cotesia plutellae bracovirus (CpBV) is a polydnavirus symbiotic to C. plutellae parasitizingyoung larvae of the diamondback moth, Plutella xylostella. Several CpBV genes play important roles insuppressing immune responses of the parasitized larvae. This study tested a hypothesis that the CpBV genesinducing host immunosuppression could be applied to develop a potent recombinant baculovirus. Based on aprevious study, a recombinant baculovirus expressing CpBV-ELP1 (AcMNPV-ELP1) was selected andmultiplied using larvae of the beet armyworm, Spodoptera exigua. The recombinant viruses were produced ina yield of 5× 10 10 polyhedral inclusion body (PIB)/larva. The cultured AcMNPV-ELP1 exhibited a muchhigher pathogenicity against S. exigua larvae. However, its insecticidal activity was varied among larvalinstars of S. exigua, in which first and late instars were high susceptible. Spray of the recombinantbaculovirus (5× 10 6 PIB/mL) exhibited higher control efficacy ( 88%) against S. exigua larvae infestingcabbage than a chemical insecticide, tebufenozide, at 7 days after treatment. These results indicate thatAcMNPV-ELP1 mass-cultured using host insect system is highly pathogenic and can be applied to develop anovel microbial control agent. Key words Baculovirus, Cotesia plutellae, Plutella xylostella, Polydnavirus, Recombinant, Spodoptera exigua
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