Cozart®RapiScan Oral Fluid Drug Testing System validation by GC-MS/MS analysis

Abstract

Introduction : The increasing number of traffic deaths related to the use of drugs of abuse has made indispensable the use of roadside tests in an easy and fast way. The test most commonly used in Spain is the immunoassay-based Cozart RapiScan Oral Fluid Drug Testing System. Due to the great number of analysis, it has become essential to validate this test by using more sensible methods. Accordingly, a test validation that combines analysis by gas chromatography and detection by mass spectrometry (GC-MS/MS) has been considered. Methods : The total number of samples examined in this study includes 216 cases collected on the road by traffic police and analyzed by a roadside test. Positive samples were confirmed by GC-MS/MS. Results : Among the 216 cases examined with the oral fluid test, 155 cases were positive and 61 were negative. In the analysis carried out by using GC-MS/MS, 158 were positive and 58 were negative. Conclusion : The results supports the reliability of the oral fluid test as a method for the detection of narcotic substances in situ , though later confirmation is recommended using a more sensitive method.