Abstract
<i>Coxiella burnetii</i>in Ticks, Argentina
Highlights
To the Editor: The Gammaproteobacterium Coxiella burnetii is the causative agent of acute Q fever and chronic endocarditis in humans worldwide
In the laboratory, engorged nymphs molted to adults (92 A. tigrinum, 13 A. parvum), which were individually submitted to the hemolymph test with Gimenez staining for detection of rickettsiaelike organisms [7]
Intracellular bacteria were successfully isolated from all 3 ticks and established in Vero cell culture, as demonstrated by Gimenez staining of infected cells from at least 10 subsequent passages, which all infected 100% of the cells (Figure, panel A)
Summary
In the laboratory, engorged nymphs molted to adults (92 A. tigrinum, 13 A. parvum), which were individually submitted to the hemolymph test with Gimenez staining for detection of rickettsiaelike organisms [7]. These 3 ticks were processed individually by the shell vial technique, with the purpose of isolating intracellular bacteria in Vero cell culture [7]. Intracellular bacteria were successfully isolated from all 3 ticks and established in Vero cell culture, as demonstrated by Gimenez staining of infected cells from at least 10 subsequent passages, which all infected 100% of the cells (Figure, panel A).
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