COVID-19 management-the Indian experience

Abstract

Extremophilic microorganisms, with their remarkable adaptability to harsh environments, hold immense potential for biotechnological and industrial applications. This study represents a pioneering investigation into the production and characterization of lipase and biosurfactants (BSs) by Halomonas zhanjiangensis. The GC-MS analysis of fatty acid profiles indicates that methyl 2-hyroxydodecanoate is the major component with 59.130%, followed by methyl 14-methylhexadecanoate (18.98%) and vaccenic acid (10.902%). The carbon sources and the composition of the culture medium were tested to optimize the lipase and biosurfactant productions. The strain Mn8 was able to produce one lipase with a molecular mass of 64 kDa. The optimum enzyme production was obtained at pH 5, pH 8, and 50 °C. The maximum BSs production obtained in the presence of LB auditioned with 1% sunflower oil. The FTIR analysis indicates that the BSs belong to the glucolipid type. The chemical composition of the BSs by GCMS shows that the major fatty acid is 2-Hendecanone C11 with 67.37%, followed by 2-Nonanone with 21.28%. The glycolipid possesses high bioemulsifying activities. In conclusion, based on the activity of the lipase at extreme conditions, associated with the bioemulsifying potential, we conclude that the novel strain Mn8 the development of industrial formulation.