Abstract
Adenovirus (Ad) vectors are produced from molecular clones (MC) of the Ad genome. E1 and E3 domains are deleted; removal of E1 prevents virus replication. The genome is cloned into a plasmid vector. Infected cells provide viral RNA, for example, spike of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Spike sequences are amplified and cloned into a shuttle vector from where the expression cassette is excised and inserted into an Ad MC. Ad MC transfection of E1+ helper cells rescues the vaccine, which is expanded, tested, and ready for good manufacturing practice (GMP) production and clinical trials.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.