Abstract
Ubiquitination plays a prominent role in cell signaling networks. Enzymes governing ubiquitination include the conjugation machinery along with the deconjugating enzymes - deubiquitases. A MspA nanopore is demonstrated as a sensor for discriminating and quantifying various ubiquitin proteins. Real-time measurement of polymeric ubiquitin chain disassembly by a deubiquitinating enzyme was performed and enzyme kinetics were determined. The implementation of MspA into arrays could enable high throughput characterizations of unknown deubiquitinases as well as drug screening against disease-related enzymes. More information can be found in the Communication by Min Chen et al.
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