Cover Feature: Measurement of Protein Dynamics from Site Directed Cu(II) Labeling (Anal. Sens. 1/2023)

Abstract

The cover feature image illustrates two sites of a protein with different site specific reorientational dynamics (purple). Such differences can be measured by newly developed site-directed Cu(II) labeling methodology. The resultant EPR lineshape at physiological temperatures is sensitive to the timescale of backbone motion. Importantly, this methodology enables site-specific detection on both α-helices and β-sheets via EPR. Thus, the role of protein dynamics to protein function can be elucidated. More information can be found in the Review by Sunil Saxena and co-workers.