Cover Feature: Affinity Selections of DNA‐Encoded Chemical Libraries on Carbonic Anhydrase IX‐Expressing Tumor Cells Reveal a Dependence on Ligand Valence (Chem. Eur. J. 35/2021)

Abstract

The screening of a DNA-encoded chemical library against carbonic anhydrase IX (CAIX)-expressing tumor cells is depicted in the cover image. DNA-encoding techniques allow the construction and screening of chemical libraries of unprecedented size, but these procedures are typically performed using purified protein targets. While the introduction of cell-based selections expanded the target scope, selection parameters remained obscure. We report systemic optimizations which translated to the successful discovery of CAIX ligands via cell-based selections. More information can be found in the Full Paper by J. Scheuermann, D. Neri, et al. (DOI: 10.1002/chem.202100816).