Abstract
AbstractMicroscopy Methods used to Elucidate Protein Adsorption and Transport Behavior on Novel Chromatographic MediaTop left: polarization anisotropy map for fluorescently labeled lysozyme in solution and adsorbed on SP Sepharose XL particles. Top right (courtesy of Dr. Harun Koku): SEM of Sepharose FF (left half) and Sepharose XL (right half), both with protein adsorbed. Bottom right: photobleaching of fluorescently labeled lysozyme adsorbed on SP Sepharose FF. Bottom left: confocal microscopy of simultaneous loading of fluorescently labeled lysozyme (green) and lactoferrin (red) on SP Sepharose FF.DOI: 10.1002/ceat.201100304B. D. Bowes, S. J. Traylor, S. M. Timmick, K. J. Czymmek, A. M. Lenhoff*Insights into Protein Sorption and Desorption on Dextran‐Modified Ion‐Exchange MediaChem. Ing. Technol. 2012, 35 (1), 91–101
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