Abstract
AbstractVascular endothelial growth factor (VEGF) type protein, a potent angiogenic effector molecule, was successfully covalently immobilized onto the surfaces of the resorbable polymers poly(L‐lactic acid) (PLLA) and poly(ε‐caprolactone) through a three‐step strategy. The surfaces were first covalently grafted with poly(acrylic acid) using non‐destructive and solvent‐free vapor‐phase grafting. A diamine spacer was coupled to the carboxylic acid pendant groups on the graft chains using 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide hydrochloride/N‐hydroxysuccinimide chemistry and VEGF was finally covalently attached to the amine linkers. The chemistry and topography of the modified substrates were quantitatively and qualitatively verified with X‐ray photoelectron spectroscopy, attenuated total reflectance–Fourier transform infrared spectrometry, UV–VIS, scanning electron microscopy, and enzyme‐linked immunosorbent assay. Copyright © 2010 John Wiley & Sons, Ltd.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
