Abstract
This chapter discusses the methods that are used to obtain the quantitative equilibrium and kinetic data for the water-addition reaction to pteridine. In the chapter, the various physical properties used in quantitative studies—namely, (1) electronic (ultraviolet and visible) absorption spectra, (2) acid dissociation constants (pk a values), (3) polarographic behavior, (4) the “blocking” effect of a methyl group, and (5) the nuclear magnetic resonance (NMR) spectra—are also discussed. The NMR spectrum of pteridine dissolved in heavy water changes steadily with time until equilibrium between the anhydrous and the covalently-hydrated molecules is reached. The spectra of the two species are quite distinct and, from the measurements of peak heights, the equilibrium ratio is readily calculated. The NMR spectra have also been used in the chapter to demonstrate the covalent addition of water and methanol to several 1,3,6,8-tetraazanaphthalenes.
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