Abstract
Refractile spores of Bacillus popilliae, the causative agent of a milky disease of Japanese beetle ( Popillia japonica) larvae, appear in liquid culture in the presence of activated carbon during the early part of the 2nd week of incubation. Forms resistant to drying occur during the 1st week when no refractile spores are seen. They usually number no more than a few hundred per milliliter and they vary in their resistance from day to day. During the 2nd week when refractile spores are present, the number of resistant forms rises to a peak in the vicinity of 100,000 per ml and then gradually decreases throughout the rest of a 3-week observation period. Refractile spores often disappear toward the end of the 3rd week. Thus, the terminal resistant forms may consist largely of slowly germinating spores.
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