Abstract
In this paper, we present the advantages and limitations of the coupling of a ring-oven-based preconcentration technique and surface-enhanced Raman spectroscopy (SERS). Three different methods to promote analyte adsorption on gold nanoparticles using crystal violet as a probe molecule were assessed. The results showed significant improvements in sampling process, selectivity, sensitivity, repeatability (less than ± 10%), and detection limits (nanomolar level) using a sample volume as small as 300 μL. Finally, the standard addition method was successfully applied to the quantitative SERS detection of adenine and guanine in calf thymus DNA after ring-oven preconcentration with a calculated value of (G + C)/(A + T) close to the literature value. This work could therefore pave the way to quantifying a wide variety of biologically relevant compounds in real-world samples via the use of a biodegradable, low-cost and disposable paper platform for SERS.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
