Coupling of Manganese Peroxidase-Mediated Lipid Peroxidation with Destruction of Nonphenolic Lignin Model Compounds and14C-Labeled Lignins

Abstract

Linoleic acid, the predominant unsaturated fatty acid (UFA) in the lipids of wood-rotting fungi, was oxidized by manganese peroxidase (MnP) from the white-rot fungusPhlebia radiatathrough a peroxidation mechanism. The peroxidation was markedly stimulated by hydrogen peroxide. UFAs that are substrates for lipid peroxidation and surfactants that emulsify water-insoluble components were essential for the MnP-catalyzed destruction of a nonphenolic β-O-4-linked lignin model compound (LMC). Moreover, both components stimulated the MnP-catalyzed mineralization of14C-labeled synthetic lignin and14C-labeled wheat straw. A high level of destruction was obtained in reaction systems with Tween 80 acting both as surfactant and source of UFAs. The presence of the linoleic acid in reaction systems with MnP and Tween 80 additionally enhanced rate and level of LMC destruction and lignin mineralization. The results indicate that lipid peroxidation may play an important role in lignin biodegradation by wood-rotting basidiomycetes and support the hypothesis of coupling between the processes.