Coupled mixotrophic denitrification and utilization of refractory organics driven by Mn redox circulation for significantly enhanced nitrogen removal

Abstract

Coupled mixotrophic denitrification and degradation of organics driven by redox transition of Mn for nitrogen removal has attracted much attention. Herein, this study explored the removal performance and mechanisms for nitrogen and refractory organics from secondary effluent in up-flow MnOx biofilter. Results showed that the removal of organics and nitrate was significantly enhanced by the synergistic process of heterotrophic denitrification and Mn(II)-driven autotrophic denitrification (MnAD), which were originated from the facilitation of Mn circulation. But nitrate removal was closely related to the types of carbon source and Mn(II) concentration. Single small molecular carbon source (glucose) performed better than mixed carbon source (humic acid and glucose) in nitrate removal process (74.8% in stage 1–2 vs. 54.1% in stage 3–5). And raising external Mn(II) concentration increased the contribution of MnAD (60.2% in stage 5 vs. 46.5% in stage 3) to nitrate removal. Furthermore, the relationship between Mn/N transformation and microbial community structure shifts revealed that the redox transition between Mn(II) and Mn(IV) promoted the enrichment of denitrogenation bacteria and functional genes, thus contributing to pollutants removal. Our studies expand the knowledge of MnOx-mediated pollutants removal processes and support the potential application of MnOx for removal of residual refractory organics and nitrogen.