Abstract
Industrial exhaust gases contributed heavily to anthropogenic CO2 emissions. In this study, a novel approach of capturing CO2 from ethylene oxide off-gas and converting it into a platform C4 chemical was established by using succinate-producing E. coli. Fermentation results showed that the acetaldehyde in the off-gas was the main factor inhibiting cell metabolic capability, thus IrrE which is a global regulator from Deinococcus radiodurans was used to improve cell resistance to acetaldehyde. A final succinic acid titer of 68.12gL−1 with a CO2 fixation rate of 4.7mmolL−1h−1 was achieved. Notably, this coupled process not only realized the CO2 fixation from ethylene oxide off-gas but also provided a potential cost-effective gas source which holds potential application for bio-based succinate production.
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